Abstract:

Phenylalanine ammonia-lyase (PAL), responsible for catalyzing the conversion of phenylalanine to cinnamic acid to finish the first step of phenylalanine pathway, was the key enzyme during the biosynthesis of rosmarinic acid. The cDNA fragment of PAL gene was successfully cloned by homology cloning method (Accession No. HQ388347.1), 399 bp and encoded 133 amino acids. It was designated as PerPAL-1. The results of amino acid sequence analysis showed that the identity of the sequence of PerPAL-1 amino acid with that of Salvia miltiorrhiza and Agastache rugosa was 96% and 95%, respectively. Phylogenetic tree analysis revealed that PerPAL-1 had closer relationship with PALs from Lamiaceae plants than those of other plants. The expression of PerPAL-1 gene was the strongest in young leaves and the weakest in stems.