Abstract:

The G418 resistance expression vector pFL61-G418 was constructed and trehalose-6-P synthase gene tps1 from the strain WT-303 of Saccharomyces cerevisiae was cloned. pFL61-G418-tps1 expression vector was constructed, and it was transformed into Pichia membranefaciens by lithium acetate method. Overexpression of tps1 gene improved the stress tolerance of P. membranefaciens, enabled the cells to increase viability and biocontrol ability. We succeeded in construction of vector for high trehalase by overexpression of tps1 gene in antagonistic yeast. The results showed that yeast shuttle vector pFL61 could be used to transform wild type antagonistic yeast and G418 resistance can be used as a dominant selectable marker to select transformants. The plasmid pFL61-G418 provides a visible and effective method for studying the functions of exogenous genes expression in wild type antagonistic yeast.