Abstract:

Full length cDNA sequence of heart fatty acid binding protein from goat was cloned by RT-PCR technology. The analysis and prediction on the amino similarity, physicochemical properties and secondary structure character were conducted from acquired sequences. The results showed that goat H-FABP gene cDNA was 402 bp in length and its homology rates of predicted amino acid sequence was 100% to sheep. H-FABP belong to hydrophilic and faintly acid protein. The secondary structure was mainly composed of βsheet. It had no transmembranous regions but possessed potential signal peptide, several other signal sites and cell fatty acid binding site. These results would provide theoretical base for revealing the protein space structure and biological function.