Abstract:

The resistance induced by RNAi to plant virus is a new strategy for controlling the viruses in recent years. In this study, according to the basic principles of RNA interference, two fragments (418 bp) of GVA MP gene were amplified with PCR and inserted into both sides of intron of pFGC5941 in forwards and reverse way so that it produces hairpin RNA during translation efficiently. The construction of the interfering vector pFGC5941-GVAFR targeting to GVA MP gene was identified by PCR, and the vector was transferred into agrobacterium EHA105 successfully, it provided an important basis for further studying its RNAi effection.