Abstract:

To prepare a polyclonal antibody (pAb) specific for Para Red. Hapten Para Red was coupled with carrier protein of cationed bovine serum albumin (cBSA) to form a complete antigen by mixed anhydride methods. The conjugates used to immunize Japanese white rabbits were identified with UV scanning spectrum and the specificity of pAb was detected by indirect ELISA and in indirect compete ELISA(icELISA). The couple rate of the antigen was 14:1, the titer of pAb was 1×106and the detection limit in term of 50% inhibition rate was 7.43 ng/ml in the linear range of 0.1ng/ml~1μg/ml. The cross-reaction rate between para red and pAb was 100% and was 97.8% between Sudan I and pAb.there was very lower cross-reaction between pAb and para red analogs. The anti-Para Red polyclonal antibody had a high specificity and was good enough for immunoassay of para red in edible food products.