Abstract:

Abstract: To construct an eukaryotic expression vector pcDNA3.1(+)-ATF4 by cloning mouse ATF4 gene and provide basis for establishment of gene function at the cellular and individual level.Extracted RNA, and using RT-PCR, and nested PCR method ,we successfully amplified coding sequence of porcine ATF4 and cloned it into the pMD18-T vector and sequencing confirmed and then constructed pcDNA3.1 (+)-ATF4 vector which was identified by restriction enzyme analysis and DNA sequencing.The pig ATF4 gene is successfully cloned and an eukaryotic expression vector pcDNA3.1-ATF4 is successfully constructed. Transiently transfected C2C12 cells and effect of expression is obvious, Which will contribute to further studies on the ATF4 function and to the establishment of condition.