Abstract:

ABC (ATP-Binding Cassette) transporters constitute the largest protein family with the most variety of functions. They are involved in the excretion, accumulation and the transmembrane transport of plant secondary metabolites. Through screening an ethylene-induced latex SSH cDNA library combined with RACE, one ABC transporter gene EST and its full-length cDNA were obtained, which was 3753 bp with an ORF that encoded a polypeptide of 1250 amino acids. The results showed that the ABC transporter gene had multiple copies in rubber tree genome and was primarily expressed in latex. Under ethylene or jasmonic acid induction, its expression was up-regulated. Base on above analysis, we speculate that the ABC transporter is closely correlated to the transport of rubber biosynthesis metabolites.