Abstract:

In this article to make a pGH and rabbit monoclonal antibody IgG anti-porcine growth hormone to affect the test elisa (enzyme-linked immunosorbent assay) system of factors, established to detect porcine growth hormone elisa method. Porcine growth hormone monoclonal antibodies optimum operation reference value: the optimum coating antibody concentration 3μg/mL, coated overnight at 4℃ condition, 5% goat serum closed 1h, rabbit IgG anti-porcine growth hormone best work Concentration 2μg/mL.