Abstract:

Adopting the wall degested with enzyme and low osmotic method to prepare the chromosome production of Chinese cabbage, some related factors effecting on the mitotic index and chromosome preparation quality were studied, including the root tip length, pretreatment medicament and time, the tempreture and time of pre-low osmotic and enzyme hydrolysis, and fluorescence in situ hybridization was also used to detect the chromosome preparation quality using the 25S rDNA as probe. The results showed that the best method was treating the root tips with 0.002 mol/L 8-hydroxyquinoline for 2 h when the root length was 1.5-2 cm, 0.075 mol/L KCl at 25℃ for 0.5 h pre-low osmosis treatment, and the optimum conditions for enzyme hydrolysis were at 4℃ for 5-6 h or 25℃ for 2-2.5 h with 2% cellulase and 2% pectinase. The high quality chromosome preparation with scattering chromosomes and clean background was achieved by above method, and could be used to FISH without needing pretreatment. This study will set up the foundation for the related research about FISH in Chinese cabbage.