Abstract:

Wild lily species is exiguous, individual species is unique to China, rapid propagation is an effective method to protect endangered or rare wild lily species. The purpose of this experiment was to develop a rapid propagation of Lilium amabile. This study used wild lily Lilium amabile as test materials, based on MS culture medium, additional different type and concentration of plant growth regulating substances (6-BA, NAA) induced adventitious bud and regenerative plants. With Lilium amabile bulb as explant, and determine the best sterilization time, bulbs affected bulb bud induction best hormone combination and different hormone of bulb buds proliferation influence. The results showed that the best disinfecting time was 0.1 % HgCl disinfection 10 minutes; Due to the different proportion endogenous hormones, the best bulb inductive medium: MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L; the best subculture medium: MS + 6-BA 1.0 mg/L + NAA 0.1 mg/L; the best proliferation medium: MS + 6-BA1.0 mg/L + NAA 0.15 mg/L.