Abstract:

The technique of chromosome doubling of haploids of Asparagus officinalis L. have been studied in this paper. In vitro chromosome doubling of haploids was studied by soaking shoot tips in colchicine solution and adding colchicine in the culture medium. The results indicated that adding colchicine in the culture medium increased the efficiency of chromosome doubling than that by soaking method. The induction rate and the survival rate of treating A. officinalis with 0.3% colchicine for 7 days by adding colchicine in the culture medium reached 82.50% and 80%. Compared with the haploid, the diploid stems showed thinker with larger guard cell and stoma and more chloroplasts.