Abstract:

To provide new marker for the identification of the chromosome of the Allium fistulosum L. and establish of molecular cytogenetic karyotype of the A. fistulosum L., this study prepared good dispersed metaphase chromosomes of A. fistulosum L.by removing the walls and flame drying, and carried out CPD (PI and DAPI combination) staining and 45S rDNA fluorescence in situ hybridization (FISH). Then karyotype analysis of A. fistulosum L. was finished according to chromosome morphology of A. fistulosum L., combined with the results of CPD staining and FISH. The result of CPD staining indicates that all ends of the chromosome hands of the A. fistulosum L. were CPD bands. The result of FISH shows that there was a pair of situs of 45S rDNA (on the 5th pair of chromosome), and the formula of A. fistulosum L. was 2n=2x=16=2sm+12m+2st (SAT)), the karyotype types was 2A. The results show that the use of CPD staining and 45S rDNA FISH not only provides new marker for the identification of chromosomes, but also contributes to understanding of the distribution of chromosomal GC rich region, providing molecular evidence for the research on the identification, classification and evolution of Allium species.