Abstract:

7 isolates with broad-spectum antagonistic activity against Apple Early Defoliation Disease were screened from 124 isolates. Strain BS24 was the most effective one with the width of inhibition zone 9.5 mm and 9.2 mm against Alternaria alternata f. sp. Mali and Marssonina coronaria (Ell Et Davis) Davis, and was identified as Bacillus subtilis. Cell-free culture filtrate could also strongly inhibit the mycelial growth and conidia germination of Alternaria alternata f. sp. Mali and Marssonina coronaria (Ell Et Davis) Davis. Strain BS24 had broad-spectrum angonistic activity against several other diseases of apple such as Phyllosticta pirina, Phyllositic solitaria, Alternaria Mali, Bolyosphoma berengeriana, Physalospora piricola and Glanerella cingulata. Strain BS24 had great potential of application in the field control of Apple early defoliation disease.