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Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (22): 156-161.

Special Issue: 园艺

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Genetic Diversity Analysis of Sugarbeet From the Northwest Region by SRAP

  

  • Received:2011-03-09 Revised:2011-03-09 Online:2011-09-05 Published:2011-09-05

Abstract:

In the present study,SRAP molecule marker was used to investigate the genetic diversity of 81 sugarbeet varieties from Northwest of China and 9 varieties from foreign in order to provide theoretical basis for parent selection of sugarbeet breeding,molecular marker-assisted selective breeding, germplasm resources and so on. 33 of 88 were obtained on basis of availability. A total of 592unambiguous bands were obtained, 324of which were polymorphic. The average ratio of polymorphic bands was 54.7%. Compute over-all mean showed that genetic distance was 0.3732, genetic similarity among varieties was and 0.6891, the genetic similarities within different groups of genotypes were 0.8364 among monogerm genotypes, 0.7528 among foreign genotypes, 0.7059 among polygerm tetraploid genotypes, and 0.6970 among polygerm diploid genotypes. The 90 genotypes were divided into three cluster groups based on cluster analysis by MEGA3.1 (at intercept of 0.2). The genotypes from Northwest region showed high level of genetic diversity.There are a definite difference between foreign and native genetic background based on genetic diversity index analysis by POPGEN32. Northwest materials are mainly high yield and medium Rhizomania resistant type.