Abstract:

AB01, the two-type line of multiple-allele inherited male-sterile Chinese cabbage (Brassica rapa L. ssp. chinensis ) was bred by our research group, the technology system of this material had been build, but its sterile molecular mechanism was not clear yet. In this research, sterility and fertility cDNA libraries including 27 differentially expressed clones were constructed using the fertile and sterile buds of AB01 by the suppression subtractive hybridization (SSH). According to BLAST screening and functional annotation, 25 ESTs were homology to known sequence of the databases at the National Center for Biotechnical Information (NCBI). The 25 genes, with homology to known proteins, could be divided into 4 groups (7 flowers developing-related genes, 5 lipid metabolism, 3 energy metabolism genes, 3 chloroplast related genes and 7 encode the unclassified proteins). The results suggested the molecular mechanism of multiple-allele-gene controlled male-sterile Chinese cabbage was related with lipid metabolism, energy metabolism and photosynthesis progress.