Abstract:

To establish the regeneration technique system of Portulaca oleracea which lay the foundation for the research of large-scale propagation and genetic improvement, the optimal conditions for disinfecting of explants, inducing of callus, differentiating of adventitious buds and rooting of test-tube seedlings of Portulaca oleracea were studied by using leaf and stem bearing buds as explants. The results showed that the optimum disinfection method was to put explants in 0.1% HgCl2 for 8 min; MS+2,4-D 0.5 mg/L+6-BA 0.5 mg/L was the best medium to induce callus, MS+6-BA 5.0 mg/L+NAA 0.4 mg/L was the best sprout medium; The optimal culture medium was 1/2MS+IBA 2.0 mg/L for rooting.