Abstract:

In order to draw a phylogenetic tree of 4 species of Cassia in China, the study used guaiacol method and polyacrylamide vertical slab gel electrophoresis to test the resistance indicators and peroxidase isozyme, and analyzed the differences between the result and traditional systematics. Peroxidase activity of 1 day, MDA of 3 days significantly differed among species, so it can be used as one of the indicators to explore their genetic relationship. 12 POD enzyme bands were found, characteristic enzyme bands are mainly in b-zone and a-zone. Differed from traditional Systematics, both resistance indicators and POD zymogram revealed that Cassia sophera is closer to C. nomame than C. alata in Systematics, while C. tora belong to another branch.