Abstract:

Timely and accurate diagnosis is the key to control foot and mouth disease.Both in the laboratory or field, ELISA is the most common diagnostic methods of FMD because it has many advantages. We introduce the principles, characteristics and research progress of conventional and novel ELISA. The conventional ELISA, including Liquid-phase Blocking sandwich ELISA, Indirect ELISA, Solid-phase Competition ELISA and Indirect Sandwich ELISA .While McAb ELISA, RT-PCR ELISA, Biotin-avidin ELISA and Dot-ELISA belong to The novel ELISA .The direction of the development of ELISA is sensitive, stable, simple and easy to automate.ELISA would be more widely used in clinical diagnosis and epidemiological investigation, with advances in technology.