Abstract:

With a view to determining variance of pathogenic enzyme and effect of ferment filtrate of Bacillus subtilis strain B68 on pathogenic enzyme, activities of the cell wall degrading enzymes (CWDEs) produced by Fusarium semitectum both in vitro and in vivo were studied. High activities of pectin esterase, pectin, pectic lyases, cellulose and no pectic hydrolases were detected after green banana fruit were infected by F. semitectum. CWDEs activities released by F. semitectum in vitro were higher than those in vivo. By way of inducing culture in vitro, secretion of pectinases (PG、PMG、PME、PGTE、PMTE) and cellulases (Cx、β-glucosidase) produced by F. semitectum in vitro was significantly inhibited by ferment filtrate of Bacillus subtilis strain B68 in various concentration compared with CK. A scientific basis was provided when control of banana crown rot was used by Bacillus agents.