Abstract:

In order to explore the functions of SERK gene on apomictic characteristics in Malus and discuss the expression characteristics of the SERK gene in the early periods of the ovary or ovule, the middle parts of the ovary from 28#, 33# (which are the offspring of Pingyi TianchaבZha’ai Shandingzi’) and Pingyi Tiancha were taken as the materials in the research. Based on the RNA extractions from all the materials and the SERK sequences obtained from leaves of Pingyi Tiancha, the specific primers were designed and 18S rRNA was taken as the internal standard, the RT-PCR analysis was carried out in the 3 different materials expression situation around florescence, The results indicated that SERK gene fragments expressed in all different materials and stages, but expression intensity displayed differences. The relationships between SERK gene fragment expression characteristics and embryonic development, apomictic features were discussed in this article.