Abstract:

To study the effect of oncogene c-fos and c-myc on velvet antler growth, the antler yield of 231 Tarim wapitis were collected. And the first-cut antler and reborn fresh velvet antlers of 3 adult Tarim wapitis with 30 or 60-day-old were used to be experimental material. Quantitative expression in different zones of antler were determined by fluorescent quantitative PCR. The results demonstrated that the expression of c-myc in reborn dermal layer of antler early growth was lower than that in the first-cut antlers （P<0.01）. The gene c-myc is down regulated in dermal layer and cartilage layer of the reborn antler with 60-day-old, and compared to 60d of natural growth antler, it were significant difference in both of them（P<0.05）. The low expression of c-myc was observed in mesenchymal cells layer and precartilage cells layer in different growth period. Dropped expression of c-fos was observed in dermal layer of reborn antler while upward expression in cartilage layer from 30d to 60d and there was significant difference compared to natural growth antler(P＜0.05).Little variation was found between the expressions of c-fos in mesenchymal cells layer and precartilage cells layer.Express of c-fos in reborn antlers with 60-day old was similar to that the first-cut antler with 30d, and c-fos is down regulated in cartilage layer of the reborn antler. The results suggested that c-myc and c-fos mainly promote the proliferation and differentiation of stem cells in dermal layer during the rapid growth of the antlers while in cartilage layer high expression of c-myc may inhibit cartilage osteoblast and low expression of c-fos inhibit process of Periosteal endogenous bone in antler’s growth period.