Abstract:

The aim was to study the effects of electron beam irradiation on pollen germination rate and provide the base for mutation breeding of electron beam irradiation. The maize pollen of ‘Beijing-waxy 28’ and pollen of Gossypium hirsutum L. were exposed in electron beam with different dose, the irradiated pollens were cultivated in a liquid medium, and then the pollen germination rates were observed and counted. The pollen germination rate of ‘Beijing-waxy 28’ was higher than that of the control group in 200-600 Gy radiation dose, with the highest germination rate at 600 Gy radiation dose. While the pollen germination rate of Gossypium hirsutum L. was lower than that of the control group in 300-1000 Gy radiation dose, but after the 50 h, the pollen germination rate was higher than that of the control groups. Electron beam radiation doses in the range of 200-600 Gy could facilitate the pollen germination rate of ‘Beijing-waxy 28’, and Electron beam radiation doses in the range of 300-1000 Gy could inhibit the pollen germination rate of Gossypium hirsutum L., but it could extend the activity of the Gossypium hirsutum L.pollen in a certain degree.