Abstract:

The aim was to establish the tissue culture technique of Salacca zalacca and provide the theoretical basis for large scale propagation of Salacca zalacca. With the embryo of Salacca zalacca as explants, the effect of plant growth regulators types and concentration on multiple shoots and root induction were studied. It took about 6 months to induce multiple shoots. The highest shoot regeneration frequency (41.7%) was recorded on MS medium with 8.0 mg/L 2ip and 0.25 mg/L NAA. Half-strength MS medium with 1 mg/L IBA and 1 mg/L ABT was best for root induction where the rate of rooting was 46.9%. The in vitro-raised plantlets with roots and height ＞5 cm were transplanted into polythene bags with yellow subsoil + perlite. The survival rate was more than 80%.