Abstract:

To establish SRAP reaction system for mini-watermelon and explore the generality of the SRAP technology system among different type of watermelon resources, the SRAP reaction system was optimized, in terms of Mg2+, dNTPs, primers, Taq polymerase and template DNA 5 factors and their 4 levels with the primer Em3-Me3, by an orthogonal design. And further application of the optimal reaction system was applied among different type of watermelon resources. The results indicated that the watermelon appropriate SRAP-PCR reaction system in a 10 μL reaction system was: 10× PCR buffer 2 μL, Mg2+ 3.0 mmol/L, dNTPs 0.2 mmol/L, primer 0.5 μmol/L, template DNA 40 ng, Taq polymerase 0.5 U. 37 mini-watermelon germplasms were used to test the stabilization of the optimized reaction system. The optimized reaction system was very stable and can well meet the different type watermelon requirements of genomic DNA amplification.