Abstract:

The aim was to reveal the mechanism of alien plant invasion on soil microbiology, the PCR-DGGE combined with cloning of V3 region of fungi 18S rDNA fragments were used to analyze the effects of biological replacement on fungi diversity of soil invaded by Flaveria bidentis, and with the changing of soil nutrient contents. The analysis of DGGE profiles showed that: fungal community structure significantly changed and the similarity between the biological replacement treatment and the no-treatment was only 45%, the index of replacement control on fungi communities’ diversity of soil invaded by the F. bidentis was only 2.04, but the no-treatment was 2.78. Besides, soil fungi diversity index of different plant communities was positively related with the content of organic matter and microbial biomass carbon, but significantly negative with phosphorus content of soil. So that the biological replacement of F. bidentis induced the changes of soil nutrient contents by changing fungi communities diversity. In conclusion, biological replacement has effect on the soil environment F. bidentis grow, and further control its spread effectively.