Abstract:

In order to improve the detection speed and efficiency, a double antibody sandwich ELISA was developed to detect Vibrio parahaemolyticus，Vibrio vulnificus or Vibrio minicus in food simultaneously. The cytorrhyctes of the fusion protein were purified by the electroeluting to immunize cavia cobaya,anti-serum were uesed as capture antibody，goat anti-guinea pig IgG/HRP were uesed as labelled-antibody,optimizing condition was determined by use of the chessboard titration method. The result showed that,the double antibody sandwich ELISA having obvious positive reaction with the culture supernatant of Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio mimicus , but no positive reaction with 8 other culture supernatant of contrast strains including Pseudomonas aeruginosa, Staphylococcus aureus, Listeria, etc. It indicated that this method can be used to detect Vibrio parahaemolyticus, Vibrio vulnificus and V. mimicus toxin in food simultaneously.