Abstract:

In the study, we are aimed to investigate the antimicrobial activity of isoalantolactone against Staphylococcus aureus, and further assess the influence of isoalantolactone on the production of α-toxin by S. aureus. The minimal inhibitory concentrations (MICs) were determined by a broth micro-dilution method. The effects of isoalantolactone on α-toxin production were assessed by hemolysis, western blot, and real-time RT-PCR assays. The MICs of isoalantolactone against S. aureus strains were greater than 1,024 μg/ml. However, low concentrations of isoalantolactone could substaintially inhibit the expression of α-toxin by S. aureus. The data present here indicate that isoalantolactone may potentially be used as a lead compound for the development of anti-virulence drug.