Abstract:

To establish a kind of simple and rapid high perfeormance liquid chromatograpy determination method of organic acid in Fu-brick tea, Kromasil C18 column was used at 17℃ and mobile phase was 3% CH3OH -0.1 mol/L KH2PO4 (pH value adjusted with phosphoric acid to 2.7) with flow rate of 0.4 mL/min. 10 μL sample was injected into the column and detection wavelength was 210 nm. The relative standard deviations were lower than 1.80%, and recovery was 90.12% to 105.74%. All of the correlation coefficients between the concentration of the organic acids and the HPLC response areas were higher than 0.998, indicating that the method was simple, rapid and accurate. At the same time, the method has been applied to detect composition and content of organic acids. Results showed that 11 kinds of organic acids were detected in fu-brick tea.