Abstract:

To study the effect of bufonis parenteral on lymphocytes, different concentration of bufonis parenteral solutions were selected as the experimental group. The BALB/c mice spleen lymphocyte incubated for 48 hours with different concentration of bufonis parenteral solution to test the level of cell proliferation. By ELISA method, the effects of bufonis parenteral on the secretion of several important cytokines (IFN-γ, IL-2 and IL-12) were evaluated. With the use of a method of flow cytometry tested the effect of bufonis parenteral solution on cell expression and cell cycle of CD4/CD8 of T-cell in mice spleen. The results indicated that bufonis parenteral solution in a certain dose range could significantly enhanced the proliferation of lymphocyte and the IFN-γ, IL-2 and IL-12 secretion; increase T-cell subset percentage of CD4+ CD8- and CD4- CD8+ phenotypes in mice spleen and the percentage of lymphocyte entering into the phase. The results indicated that the bufonis parenteral solution could significantly increased the lymphocyte proliferation and function in vitro, concluded that bufonis parenteral solution has significantly enhanced specific immunological function.