Abstract: The recombinant retroviral vector pBABE-puro-E2 was constructed by inserting full-length cDNA of CSFV Shimen strain E2 gene into pBABE-puro. Both the recombinant retroviral vector and pVSVg plasmid were transfected into eukaryotic cells 293GP by calcium phosphate transfection method thus the pseudovirus were producesd . The pseudovirus infected eukaryotic cells SP2/0 and expression E2 protein was determined by puromycin-resistant and FACS analysis. BALB/c mice were intraperitoneal injected with SP2/0 cells which expressed CSFV E2 protein. The result showed anti-CSFV E2 antibody was screened by FACS.