Abstract: The variation in the relative enzymes activities and lignin content in leaves and stems/roots of four hot pepper seedlings induced by SA and after challenge inoculation with Phytophthora capsici were studied. The results shows that the lignin content and activities of PPO, POD and PAL in four hot pepper lines treated with 0.225g/L SA were increased compared with untreated plants, and the lignin content had a significant positive correlation with varietal resistance of pepper to P capsici. The lignin content and activities of PPO, POD and PAL in pepper seedlings by SA-treatment were much higher than that in untreated control after challenge inoculation with P capsici, and this kind of increment came more rapid and earlier in the resistant and tolerant pepper lines than in susceptible and high susceptible ones. After challenge inoculation, the lignin content was also significantly positively correlated with the disease resistance in SA-treated pepper plants, which suggested that the variation of lignin level, PPO, POD and PAL activities corresponded well with the induced resistance against P capsici in pepper plants by SA-treatment.