Abstract:

In this experiment, it was aimed to investigate the suitable primary in-vitro cultured methods for the uterine endometrial cells of dairy cattles and sheep. Total four methods were compared with one another as following: the method by tissue culture only, the method by normal trypsin digestion only, the method by tissue cultured-trypsin digestion, and the one by trypsin digestion—tissue culture. The results showed that the uterine endometrial cells of dairy cattles and sheep took shape of either the spindle cell or the cobblestone morphology cell, normally grew faster than the method by tissue culture only than those by the other ones. The uterine endometrial cells of dairy cattles could not be obtained from the method by normal trypsin digestion only, but the uterine endometrial ones of sheep. Contrastly, through the method by the tissue cultured-trypsin digestion, the bovine uterine endometrial cells could be obtained, but could not be passaged and survival for a relatively long duration. In addition, through the method by the trypsin digestion-tissue culture, both kinds of uterine endometrial cells could be obtained similarly to the one by tissue culture only, but it easily displayed the residual block overlaying on the newly spreading cells, which would inhibit those viable cells from their proliferation. Collectively, it can be concluded that the method by tissue culture only is a shortcut, highly efficient way for establishing the in-vitro cultured model for the uterine endometrial cells of both dairy cattles and sheep. Such kind of uterine endometrial cells can be passaged for at least 4 generations, and be applied for the approriate analysis about their cellular function and molecular regulation.