Abstract:

In order to explore a method to fast extract high-quality soybean leaf genomic DNA. This study improved buffer composition and concentration, added surfactant Tween-20, and optimize the experimental procedure on the basis of the SDS method. The extracted DNA was digested with the restriction enzyme EcoR I, electrophoresis displayed it was digested completely. And the DNA was used as template to amplify the soybean Actin gene, sequencing confirmed the segment was correct. And the DNA was also used for SSR-PCR reaction with SSR primer Sctt011, polyacrylamide gel electrophoresis results showed the bands were specifically and clearly. The results show that the extracted DNA can be used for various molecular experiments. The method can shorten the DNA extraction time, while improve the quality of extracted DNA.