Abstract:

In order to identify the concentration of ammonium while screening the positive mutants of soybean transformed by particle bombardment, transferred the embryonic tips of mature soybean ‘Hedou 12’ to the MS medium supplemented with different concentrations of ammonium. It identified that, the concentration of ammonium was the condition of screening the mutants, which could inhibit the growth of embryonic tips. The growth of embryonic tips was not effectively inhibited, when the concentration of ammonium was lower than 0.5 mg/L. When the concentration of ammonium was higher than 0.5 mg/L, the plants were significantly inhibited and could not regenerate after transition to MS medium. So, 0.5 mg/L was the concentration for biolistics transformation of soybean [Glycine max (L.) Merrill cv ‘Hedou12’].