Abstract: This experiment was done in the 26 years fixed position experiment of non-calcareous fluro-aquic soil during the year of 2003，12 treatments were designed: CK、N1、N2、M1、M1N1、M1N2、M2、M2N1、M2N2、NPK、NP and NK. The soil water holding and humus constitution were studied. The results indicated that: soil porosity of every fertilization treatment in the depth of 0~20cm soil were all higher than that of check, the treatment of using organic fertilizer and inorganic nitrogen fertilizer were 11.49%~19.24% times higher than check, the soil water content was positively related to soil porosity (r=0.7076*). Combined application of organic fertilizer and inorganic nitrogen fertilizer can increase organic-C, humic acid-C,Fulvic acid-C,humic-C. Application of organic fertilizer (contain 18.4kg N) and combined application of organic fertilizer and inorganic nitrogen fertilizer (contain 18.4kg N) were 73.45%, 198.58%, 140.85% and 39.35% times than that of the treatment of non- application of organic fertilizer. Application organic fertilizer for long time can improve the content of humus, and inorganic fertilizer can enhance the content of Fulvic acid, decrease the content of humic acid. From above A combined application of organic fertilizer and inorganic fertilizer can improve soil physics properties and the ability of soil available nutrients and water-holding capacity.