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Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (17): 75-81.doi: 10.11924/j.issn.1000-6850.2011-2935

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Optimization of Preparation Technology of Medicated Serum of Yinqiaotiangan in Mice by Response Surface Methodology

  

  • Received:2011-10-11 Revised:2012-01-18 Online:2012-06-15 Published:2012-06-15

Abstract:

In order to optimize preparation technology on medicated serum of Yinqiaotiangan in mice, on the base of single factor investigation, taking-blood time, administration times, administration concentration were selected for the independent variable and comprehensive score on the concentration of chlorogenic acid and forsythia in the mice serum was as response value. Effects of every independent variable as well as their interactions on response value were studied by response surface analysis. The predictive model of two polynomial regression equations was analyzed by Design-expert software. The results showed that preparation technology was concluded as take blood time 30 min, administration times 4 times and administration concentration 0.02 g/mL. In theoretical calculation, comprehensive score was 95.62%. In this condition, the concentration of chlorogenic acid was 33.65 μg/mL and concentration of forsythia was 193.86 μg/mL in mice serum. This study provided the foundation for preparation technology on medicated serum of Yinqiaotiangan.

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