Abstract:

The strach branching enzyme (SBE) can introduce α-1,6-glucan linkages to α-1,4-glucan, which make the starch brached. There are three isoforms of SBE: SBEⅠ, SBEⅡa and SBEⅡb. Many studies were focused on SBEⅡb, and few studies on SBEI. In order to clarify the role of SBEI in starch synthesis, the 337 bp fragment of SBEⅠ gene was amplified based on the sequence AF07272, and used the pMD19-T vector as interim vector the RNAi hairpin of SBEⅠ gene was constructed into pCambia3301 vector, and the new vector was named as pC3301-SBEⅠ. Through the identification of restriction endonuclease, the pC3301-SBEⅠ was proved to be correct. The new vector was used to transfer maize mediated by agrobacterium tumefaciens.