Abstract:

In order to solve the problem of extracting total RNA from grape tissues, the author took the leaf-roll associated virus 3 infected grapes varieties ‘Gernischt’ as materials, the method of CTAB combined with SDS was studied in this paper, a suitable method for total RNA extraction from leaves, petioles and phloem of grapevine was established. The results indicated that high-quality RNA could be obtained by this method, with OD260/OD280=1.8-1.9, without degradation. The results showed that the RNA obtained could be used for RT-PCR to detect grapevine viruses. The method was simple, the results were stable. It also gave better suggestions to extract total RNA of tissues which rich in polyphenol and polysaccharides.