Abstract:

In order to study the Population Subdivision and genetic variation of Fusarium oxysporum f. sp. cubense in Zhuhai of Guangdong Province, amplified fragment length polymorphism (AFLP) was used to analyze the DNA fingerprint of 20 different isolates of Fusarium oxysporum from Zhuhai, Zhanjiang and Taishan. One isolate of F. equiseti (FOC017) from tissues of Musa ABB was chose to be biological control. 17 isolates of Fusarium oxysporum were from Zhuhai, including 12 Foc race 4 isolates, 2 Foc race 1 isolates and 3 non-pathogenic isolates. Two Foc race 4 isolates from Zhanjiang and one Foc race 1 isolates from Taishan. The results showed that a total of 253 AFLP bands were obtained using 8 primer combinations, and 97 of them, covering 38.34%, showed polymorphism. Genetic distance of 21 isolates ranged from 0.41 to 0.95, averaged 0.85. Results of UPGMA analysis divided 21 tested isolates into three major groups, which was race 1, race 4 and non-pathogenic Fusarium oxysporum. AFLP analysis revealed that the diversity level of race 1 was greater than that of race 4 and there was a correlation between AFLP groups and geographic origin of tested isolates. The results indicated that the genetic diversity of F. oxysporum f. sp. cubense was high among tested isolates from Zhuhai.