Abstract:

To study the effects of Soy Isoflavones on lymphocyte proliferation in spleen and lymph nodes of Holstein cows, investigate the mechanism to improve immune performance of cows. The experiments were assigned into 7 groups: control group (blank), 100.00, 25.00, 5.00, 1.00, 0.25, 0.05 μg/mL, Soy Isoflavones and incubated with lymphocyte to 48 h or 60 h, respectively. The plates were read at 570 nm. The results showed that: First, for the lymphocyte of lymph nodes, all over the concentrates of Soy Isoflavones ranging from 0.05 μg/mL to 25 μg/mL had promoted effect to the lymphocyte proliferation, while the most promotion concentrates was 5 μg/mL it reach the most promotion effect. But when the addition concentrations of Soy Isoflavones enhanced reached to 100 μg/mL, the inhibition suppressed effect of lymphocyte proliferation was significantly; Second, in lymphocyte of splenocyte, the lymphocyte proliferation at higher concentrations (100 μg/mL and 25 μg/mL Soy Isoflavones group) had a significantly suppressed effect on the proliferation of lymphocyte in the spleen of the cow (P<0.01). (P<0.01) comparing with the control. However, at lower concentrations (1 μg/mL) the lymphocyte proliferation could be significantly stimulated (P<0.05). The other groups had the same inhibition suppressed effect, but the difference was not significant.