Abstract:

In order for the industrialized rapid propagation and expanding cultivation of Dalbergia odorifera and lay a certain foundation for the introduction of cold resistance genes, optimal medium composition for callus induction and differentiation was investigated, with stems, leaves, root apexes of sterile seedlings as explants and MS as basic medium. The results showed that the callus culture and plant regeneration from the various organs were successfully achieved. The optimal media for callus induction from stem segments, leaves, root apexes of sterile seedlings were 1/2MS+6-BA 1.5 mg/L+NAA 0.10 mg/L, 1/2MS+BA 0.5 mg/L+NAA 0.10 mg/L and 1/4MS+6-BA1.5 mg/L+NAA0.05 mg/L, respectively. The optimal media for the proliferation of clustered shoots from the callus inducted from the three organs were 1/2MS+6-BA 1.5 mg/L+2,4-D 4.0 mg/L, 1/2MS+6-BA 1.5 mg/L+2,4-D 3.5 mg/L and 1/2MS+6-BA 1.5 mg/L+2,4-D 3.5 mg/L, respectively. The best rooting media for single shoots from the callus of the three sources were MS+NAA 1.5 mg/L, MS+NAA 1.0 mg/L and MS+NAA 2.0 mg/L, respectively. In light of the rates of callus induction, proliferation of clustered shoots and rooting, the best system of callus culture and plant regeneration of Dalbergia odorifera was established with the root apexes of the sterile seedlings as explants.