Abstract: To study the effects of water extract from different Organic Matter on growth of regenerated shoots from Dendrobium candidum Wall. ex Lindl. In the same culture condition, the regenerated shoots were cultured on MS medium with water extract of eight Organic Matter, the different growth status was scored and general analysed after 60d. Results There were different influence to the regenerated shoots that culture media with different water extract of Organic Matter. MS adding CH, CM or water extract of white radish was the better media for strengthening regenerated shoots, while MS with the water extract of banana was suitable for root growing, MS with nothing was the best media for propagation.