Abstract:

To solve the problem of optimal use of macroporous resin purification persimmon polysaccharides, using three factors and three levels of response surface method, as the index of dynamic adsorption and desorption rate, on the basis of experiments about screening 8 kinds of macroporous resin, finally determined, the optimization results were: resin AB-8 had best separation purification of persimmon polysaccharides. The final optimization of the dynamic adsorption experiment conditions: the sample liquid concentration 4.34 mg/mL, sample rate 1.89 BV/h, sample volume 3.41 BV (column volume); desorption test conditions: eluting agent ethanol concentration 55.41%, the elution rate 3.50 BV/h, elution dosage 3.54 BV (cylinder volume). Under this condition, the dynamic adsorption and desorption rate of the predicted value respectively were 89.87% and 96.59%, the testing experiment showed the relative error between them was about 5%.