Abstract:

To study the sequence length in Intron-I of adipocyte fatty acid-binding protein (A-FABP) gene, the polymorphism and homology of the other species. A pair of primers were designed to amplify the Intron-I of goose A-FABP gene, according to the sequences of A-FABP mRNA in goose (AF472610) and A-FABP gene in chicken (AY675941) and the genetic polymorphisms of Intron-I of goose A-FABP gene were investigated by PCR-SSCP and sequence alignment. The results showed that sequence size of Intron-I of goose A-FABP gene was 1473 bp, which showed the highest homology with duck (up to 94.42%) and the homology with chicken (up to 81.82%) when aligned with same gene sequence of 12 other species; Genetic polymorphism analysis by SNPs showed that the frequencies of two alleles A and B were 0.597 and 0.403 respectively, polymorphic information content (PIC) was 0.365, genotype distribution of which was in Hardy-Weinberg equilibrium (χ2=0.02, P>0.05); the frequencies of two alleles D and E were 0.483 and 0.517 respectively, PIC was 0.375, genotype distribution of which was not in Hardy-Weinberg equilibrium (χ2=6.782, 0.01