Abstract:

To study the sequence length in MC1R gene of Reddish-brown Wusuli Raccon Dog, the polymorphism and homology of the other species. In the experiment for research object, that had 8 wild Wusuli raccoon dog, 7 reddish-brown Wusuli raccoon dog, 6 Jilin white raccoon dog and 6 silver-black fox from Qingdao Lacy breeding base. By the SNP testing, we can analysis the correlation of MC1R gene expression level in the Wusuli raccoon dog in different colors. As known as NCBI sequence (GenBank: HM852533.1), with the extraction of raccoon dog hair follicle DNA as a template, we designed primer, sing for getting MC1R gene which play an important role in mammals melanin cell synthesis melanin process. The results showed that we was successful to get the reddish-brown raccoon dog MC1R gene sequence for 1329 bp, and the known sequence homology 91.47%, 18 mutations; with the wild type Wusuli raccoon dog homology reached 99%, 8 mutations. Through the enzyme cut in MC1R genes, two genes were got enzyme type AB and BB type about 300 bp, reddish brown raccoon dog for AB, wild type raccoon dog for BB type. According to the result of the experiment MC1R genes that there were brown coat of related functions mutations, which can lead to coat mutations.