Abstract:

In order to establish efficient cutting technique system, contents of 4 kinds of endogenous hormones (IAA、GA、ABA and ZR) in Acer truncatum Bunge cutting were determined with ELISA, and physiological and biochemical mechanism of rooting during cutting was studied. The results showed that contents of the endogenous hormones were closely related to rooting during cutting in Acer truncatum Bunge. Endogenous IAA at higher concentration level and endogenous ABA at lower level helped for formation and growth of root primordium in cuttings, and endogenous GA at higher level inhibited formation of adventitious root in cuttings. During different cutting periods, the functions of endogenous ZR were different, lower concentration promoted differentiation and formation of root primordium, while higher concentration benefited to growth and development of root primordium; initial contents of four endogenous hormones had different correlations with rooting rate, those of IAA and ZR were positively correlated, while those of ABA and GA were negative correlated, in which IAA was most closely with rooting rate.