Abstract:

To understand the Fusarium oxysporum f.sp. cubense race 4 (FOC4) Pectate lyase (PL) gene sequence features, a pair of primer that according to the homologous gene sequence documented in GenBank was designed to amplify the PL gene. Using PCR and RT-PCR, the genome and open reading frame (ORF) from FOC4 was successfully amplified and named it pl2-FOC4. The gene included 3 introns and 4 exons. The cDNA encoding region was composed by 831 bases and encoded 276-amino acid polypeptide with 30271.9 Da of calculated molecular weight and 5.06 of pI. PL2-FOC4 got five N-glycosylation site, four Protein kinase C phosphorylation site, five Casein kinase Ⅱ phosphorylation site and three N-myristoylation site. The protein with evolutionary conservative and got the closest relatives with Fusarium oxyporum.