Abstract:

The purpose of this paper was to obtain hydroxyphenylpyruvate reductase gene involved in rosmarinic acid (RA) biosynthesis pathway from Perilla frutescens. Gene special primers were designed based on sequences blast of HPPR genes from other species, and the fragment of HPPR gene in Perilla frutescens was successfully cloned by homology cloning method. The gene fragment, designated as PfHPPR (GenBank accession No. HM152567.1), was 426bp encoding 142 amino acid protein. Sequence alignment revealed that the deduced amino acid sequence of PfHPPR was 92%, 93% and 92% identical to Coleus blumei, Salvia officinalis and Salvia miltiorrhiza, respectively. The PfHPPR expressed in all the tested tissues but at different levels with the highest in leaf, the second in stem and the lowest in root using the semi-quantitative RT-PCR analysis. Further expression analysis reveals that the PfHPPR transcript levels might be influenced by the signaling components and stimulus such as ABA, SA and UV-B signaling pathways.