Abstract:

In order to develop a rapid and practical method for varietal purity testing in bitter gourd (Momordica charantia L.), four bitter hybrids were subjected to Ultrathin-layer isoelectric focusing electrophoresis (UTLIEF) and field grow-out test. Protein marker bands were found in all parent lines of the four hybrids, and the protein electrophoresis of these four F1 hybrids contained both male marker bands and female marker bands. The results of UTLIEF tests and grow-out test were compared, and the results were identical. This examination subjects that UTLIEF could be employed as a rapid and practical method for varietal purity testing in hybrid bitter gourd.