Abstract: The development of nuclear transfer embryos derived from fetal fibroblasts including t-PA gene and ovary granulose cells were compared. The cleavagetate （82.9%）and Morulae rate （55.9%）of ovary granulose cells were more high than those of fetal fibroblasts(75.7%,48.7%). Blastocyst rate (19.7%) of ovary granulose cells was significantly higher than that of fetal fibroblasts (11.0%). The development of nuclear transfer embryos derived from fetal fibroblasts including t-PA gene in media DMEM/F12 including 0.5%FBS for starvation and including 10%FBS were compared. The cleavagetate(75.6%) of nuclear transfer embryos in media DMEM/F12 adding 0.5%FBS is not significantly defferenter than that (75.8%)in media DMEM/F12 adding10%FBS. Morular rate (46.5%) and blastocyst rate (11.0%) in media DMEM/F12 including 0.5%FBS were higher than those (39.3%, 9.0%) in media DMEM/F12 adding10%FBS （P＞0.05）. The blastocyst rate(9.1%) nuclear transfer embryos in media SOF adding 10% bFF was higher that(4.2% )10% FBS. The blastocyst rate(11.2%) nuclear transfer embryos in media SOF adding 10% bFF was higher that(5.5% )10% FBS. SOF was better than CR1 for the early development in nuclear transfer embryos.